In vitro multiplication of Pothos tener shoots

Abstract

Abstract South Sulawesi-endemic Pothos tener can be used as a raw material for medicine because it contains antimicrobial compounds. To date, plants have been collected directly from the wild, without any cultivation or propagation measures. This will threaten its continued existence. Micropropagation is an efficient method for propagating plants. Multiplication of shoots is an essential step in micropropagation. Explants can be cultured on media containing cytokinins to increase the number of shoots that serve as new plant material. This study investigated the effect of cytokinins on the in vitro multiplication of Pothos tener shoots. The growth medium utilized was MS media (1962), which was added with BAP, Kinetin, 2iP at concentrations ranging from 0 to 2.5 mg/l. Every treatment was administered 10 times. This research indicates that the rate of shoot multiplication increases as cytokinin concentrations rise. However, the best results were obtained on MS media added with 2 mg/l BA, as shoot height, number of shoots, and number of leaves were all increased. At a concentration of 2.5 mg/l, fewer shoots were produced, and their growth was stunted.