Salsola collina ethyl acetate extract alleviates diabetic gastroparesis possibly through oxidative stress inhibition

Abstract

Abstract Objective: To investigate the therapeutic significance of ethyl acetate extract of Salsola collina (EES) on diabetic gastroparesis (DGP) and its underlying mechanisms. Methods: The composition of EES was analyzed by HPLC and LC/MS. A DGP model was established by streptozotocin injection and irregularly feeding a high-sugar, high-fat (HSHF) diet. Serum nitric oxide (NO), total cholesterol (TC), and triglyceride (TG) and the gastric superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), and glutathione peroxidase (GSH-Px) concentrations were measured by colorimetry and ELISA. The expression of neuronal nitric oxide synthase (nNOS) and protein gene product 9.5 (PGP9.5) in the gastric tissue were examined by Western blot and immunohistochemistry. Results: EES promoted gastric emptying delayed by DGP, which was mainly composed of 10 organic acids. Furthermore, EES increased serum NO, decreased glucose, TC and TG, increased gastric SOD, CAT, and GSH-Px, while decreased MDA, increased nNOS and PGP9.5 expression in the gastric tissue, and showed a concentration dependence. Conclusion: EES promoted gastric emptying in the DGP rats, which might be related to its inhibition of oxidative stress and the associated increase in the gastric neuron population, as well as its hypoglycemic and lipid-lowering activities. These findings suggest that Salsola may have potential benefits in the treatment of DGP.