The Antibacterial Activity of Biosynthesized Silver Nanoparticles from Tribulus terrestris Against Pseudomonas aeruginosa

Abstract

Abstract The current study’s goal is to employ Tribulus terrestris aqueous floral extracts to synthesize silver nanoparticles (AgNPs) and test their antibacterial efficacy against Pseudomonas aeruginosa (P. aeruginosa) isolated from otitis externa in dogs. Biological nanoparticle synthesis is gaining popularity over chemical and physical processes due to its cost-efficiency and environmental friendliness. Silver nanoparticles were made by mixing Tribulus terrestris plant extract with 1 mM silver nitrate (AgNO3) solution (AgNPs). UV-Visible spectrophotometry revealed the biosynthetic nanoparticles’ maximum absorbance peak at wave lengths (410nm). The SEM was spherical and homogenous in shape, with a size range of 20 to 25 nm. The XRD indicated that the size of AgNPs was 50.21 nm. EDS was utilized to determine the presence of silver element. The AFM revealed the three-dimensional structure of silver nanoparticles with a diameter of 60.17 nm on average. The Zeta potential of the particle size analyzer was 45 mV. It was observed that as the concentration of AgNPs Increased, so did the size of the inhibition zone; (150μg/ml) exhibited a larger inhibition zone (32mm) than when using concentrations of 100μg/ml, 50μg/ml, and 25μg/ml, respectively. In conclusion, biosynthesized AgNPs generated from Tribulus terrestris demonstrate potent antibacterial activity against Pseudomonas aeruginosa isolated from otitis externa in dogs.