The quality of Bali bull sexed sperms at different incubation time using egg white sedimentation method

Abstract

Abstract The success rate of Artificial Insemination (AI) can be increased by using semen from superior bulls of the desired sex. For meat production purposes, sexed semen used is the male sex, but for milk production, then the female sex is used. This study aimed to determine the effect of incubation time for sexing the sperms using egg white albumin sedimentation method on the quality of Bali bull semen. This study was using a completely randomized design (CRD) with three different incubation time treatments; 25 (T1), 30 (T2) and 35 minutes (T3) with three replications. T0 was used fresh semen as a control without incubation time. The variables measured in this study were the motility and concentration of the sperms before and after sexing. The results of this study showed that the incubation time did not affecting the sperms motility and concentration (P> 0.05). However, motility of the X sexed sperms in T2 is likely higher than in T1 and T3. Contrary, motility of Y sexed sperms in T1 had higher than in T2 and T3. It can be concluded that the incubation time during separation of X and Y sperms using egg albumin sedimentation had no significant effect on sperms motility and concentration.