Isolation and selection of proteolytic lactic acid bacteria from colostrum of dairy cattle

Abstract

Abstract Lactic Acid Bacteria (LAB) are bacteria that produce lactic acid and are very useful bacteria, one of which can act as a probiotic because it can produce bacteriocins and produce peptide compounds with high biological activity. Colostrum is the first fluid that comes out of the udder of the mother cattle after giving birth which is very good for newborns because it contains antibodies. This research aimed to isolate, selection and identify the types of LAB found in dairy cow colostrum, especially LAB which have superior proteolytic activity. LAB obtained is expected to have a positive effect on livestock productivity. This research was conducted In-vitro in a laboratory. The research procedure included the isolation of LAB from the colostrum of dairy cows, then the selection procedure by looking at the best protease enzyme activity qualitatively and quantitatively, and testing for resistance to low pH. Lactic acid isolation, selection, and identification were analyzed using descriptive analysis and quantitative analysis using one-way ANOVA for a completely randomized design. The results obtained 6 superior proteolytic LAB isolates based on the protease enzyme activity coded LAB pro 4, LAB pro 3, LAB pro 8, LAB pro 9, LAB pro 21, and LAB pro 20.