Association of the OLR1 Gene of the 3’UTR g.8232(A/C) genotypes on milk fatty acid components in Holstein Friesian

Abstract

Abstract Dairy cattle produce milk with a high proportion of saturated fatty acids (SFAs), instead of a low proportion of poly unsaturated fatty acids (PUFA). Oxidized low-density lipoprotein receptor 1 (OLR1) gene plays a key role in reducing oxidized low-density lipoprotein (oxLDL) involved in injury, dysfunction, and inactivation of endothelial cells. Association of the OLR1 gene in the 3’UTR of the g.8232(A/C) genotypes on 21 types of the milk fatty acids of Holstein Friesian (HF) cows were analysed kept at a breeding station in Central Java. Genotyping was analyzed by PCR-RFLP method using Pst1 restriction enzyme. Genotypic data were calculated for allele frequency, genotype frequency, and heterozygosity values. Effects of genotypes on individual fatty acid components were analyzed by General Linier Model. Genotyping resulted in three genotypes of AA, AC and CC with the frequenciest successively 0.275, 0.525, and 0.200; and two alleles for A (0.463) and C (0.538). Genotypes affected medium-chain SFAs for Myristic (C14:0) and long-chain for Palmitic (C16:0) and Stearic (C18:0). The AA genotype generated the lowest levels of myristic and stearic, instead of the highest content of palmitic. Further the AA genotype resulted in the highest level of Oleic (C18:1) of MUFA. Therefore the OLR1 gene of the 3’UTR g.8232 (A>C) SNP is possible considered as a molecular-based selection in reducing SFAs (myristic and stearic) and increasing MUFA (Oleic) in domestic HF cattle.