The quality of frozen semen of Etawah crossbreed buck after washing by centrifugation

Abstract

Abstract This study aimed to determine the effect of washing sperms using the centrifugation process on the semen quality of Etawah crossbreed (Peranakan Etawah, PE) buck. This study used one PE buck, in which the semen was collected 4 times by using an artificial vagina. The parameters measured in the study were the percentage of motility and viability of the sperms using Computer-Assisted Sperm Analysis (CASA). The semen was washed using Andromed and centrifuged at 3000 rpm for 20 minutes. Andromed was used as an extender to increase the volume of semen based on sperms concentration in the straw. The concentration of sperms was divided into three treatment groups; group 1, the concentration of sperms was 100×106 cells/straw, group 2 was 150×106 cells/straw, and group 3 was 200×106 cells/straw. The study was arranged based on a completely randomized design with three treatments and four replications and analyzed using ANOVA. The results of this study showed that the percentage of individual motility of the sperms after thawing on the three treatments did not differ significantly (P>0.05), with the values were 79.2%, 77.5%, 84.4%, respectively. The progressive motility was 63.2%, 64.4%, and 73.9%, respectively, and the viability was 84.6%, 84.6%, and 84.6%, respectively. It can be concluded that the quality of Etawah crossbreed frozen semen after washing using centrifugation and at different concentrations in the straw was able to maintain the motility and viability of the semen after thawing.