Gayo horse’s estrogen concentration after pospartum silent heat therapy using intramuscularly vitamin A, D, E

Abstract

Abstract Silent heat is a condition in which livestock does not show symptoms of heat but physiologically estrus occurs due to low concentrations of the estrogen. Vitamins are small organic compounds that are needed by horses for the process of development, reproduction, and activity. Vitamin preparations are easily obtained and economically for breeders. This research aimed to observe differences in estrogen concentrations in postpartum silent heat gayo horses before and after therapy using intramuscularly vitamins A, D, E. Four of six gayo mares suspected of postpartum silent heat after diagnosis using ultrasound were used in this study. Four gayo mares were divided into two groups, the control group (K1, n=1) was only injected with NaCl and the therapy group (K2, n=3) was injected with vitamins A, D, E intramuscularly twice with an interval of one week. Blood plasma samples were collected before and after therapy for 7 days, then stored in the freezer at -20°C for analysis of estrogen concentrations using EIA. Data before and after therapy were analyzed using paired sample t-test and data between groups were reported descriptively. The results showed that the estrogen concentration of postpartum silent heat gayo horses in K1 was not significantly different (P>0.05) before and after NaCl injection (251.71±57.90 vs 255.56±84.14 pg/ml), while there was a significant difference in K2 (P<0.05) before and after vitamin A, D, E therapy (194.82±56.64 vs 557.11±96.55 pg/ml). Descriptively, there were differences in the concentration of the hormone estrogen in K1 and K2 after postpartum silent heat therapy. The conclusion is postpartum silent heat therapy using vitamins A, D, E in gayo horses can increase the concentration of the hormone estrogen in the estrous phase which has an impact on the appearance of estrus symptoms that is more clearly and easily observed by breeders.