Evaluation of the Efficacy of the Inoculum Prepared from the Two Local Isolates enterobacter colacae and enterobacter ludwigii on Soybean (glycine max l.) Growth and Yield in Gypsiferous Soil under Different Molybdenum Levels

Abstract

Abstract With the aim of reducing chemical fertilizers and relying on environmentally friendly natural biofertilizers that promote plant growth and increase yield, especially in gypsiferous soils spread in arid and semi-arid regions, which suffer from physical, chemical, fertility, and biological problems, which in turn affect the density of microorganisms, This experiment was conducted to study the efficiency of the inoculum prepared from the bacteria E. cloacae and E. ludwigii isolated from gypsiferous soils and molecularly characterized in improving the growth and yield of soybeans under different levels of molybdenum in gypsiferous soil, possibly due to its absence, particularly the bacteria responsible for nitrogen fixation. The results showed that the two treatments inoculated with E. cloacae and E. ludiwgii were significantly superior to the uninoculated treatment in all studied growth and yield traits and the percentage of nitrogen and protein in the seeds, and that the treatment inoculated with E. cloacae bacteria was superior to the treatment inoculated with E. ludiwgii bacteria in all traits, as well as the treatment fertilized at the level of 2 kg Mo ha-1 was significantly superior to the non-fertilized in all traits and showed The results of the interaction between inoculation and fertilization with molybdenum showed that the E. cloacae + 2 kg Mo ha-1 treatment was superior to the comparison treatment in all traits and gave values of 278 pod plant-1, 127.66 g plant-1, 6.23 tons ha-1, 6.27%, and 39.13%. 0.36%, 0.071% for the number of pods, weight of pods, grain yield, concentrations of nitrogen, protein, and molybdenum in the seeds, and the concentration of molybdenum remaining in the soil after harvest, respectively, compared to the comparison treatment that gave the values 150.67 pod plant-1, 68.33 g plant-1, 3.43 tons ha-1, 5.42%, 33.83%, 0.33%, and 0.068 for the aforementioned characteristics, respectively.