Author:
Dakhil Ahmed Jebur,Al-Shammari Karrar Imad Abdulsahib
Abstract
Abstract
This experiment was conducted to explore the effect of fenugreek seeds (FSP) and synbiotic (SP) powders in diet as antioxidant factors on antioxidative, physiological, immunological indicators of serum and anatomical properties during exposing to the oxidative stress (OS) induced by hydrogen peroxide (H2O2) in drinking water of broiler chickens from 2nd until 35th day of age. Totally, 300 chicks Ross 308 at one day old of age were randomly allocated into five treatments with three equal replicates in each treatment (20 chicks/replicate). The treatments were distributed into negative control without feed additives (T1) whereas T2 was adding 1 ml (0.5%) of H2O2 per 1 L of drinking water as stressed positive control. The dietary additive (0.5%) each of FSP and SP was added in stressed groups (T3 and T4), respectively and dietary combination of 0.25% both FSP and SP was added in stressed group (T5). The results referred that T3, T5 and T1 registered an increased level in catalase (p≤0.05) and glutathione peroxidase (p≤0.01) activity and there was increased amounts in superoxide dismutase and packed cells volume in T4, T5 and T1 (p≤0.01) in comparison to T2. The T3, T4, T5 and T1 achieved a decreased malondialdehyde (p≤0.05) with considerable decrease (p≤0.01) in heterophils/lymphocytes ratio, uric acid, creatinine, alanine and aspartate aminotransferases compared with T2. In comparison to T2, all stressed treatments and T1 had high antibody titers against Newcastle disease (p≤0.05) and infectious bursal disease (p≤0.01). Moreover, high proportional weights of lung and heart (p≤0.01) were in T5 whereas T1 achieved high (p≤0.01) kidneys weight and large intestine length. Thus, dietary FSP and SP supplement added synergistically or individually could promote antioxidant status, regulate liver and kidney function, and improve immune system of stressed chickens.