Effect of Alcohol Extract, Volatile Oil and Alkaloid Isolated from Capsicum frutescens L. Fruits on Candida albicans

Abstract

Abstract This study involved extracting the of alcohol extract, volatile oil and alkaloid isolated from Capsicum frutescens L. fruits on Candida albican,. It also involved test a phytochemical screen and determine the percentage yield of the alcohol extract, volatile oil and alkaloid, Capsicum annuum L. was collected from the Karbala City, that approximately 24 hours to extract using Soxhlet apparatus with 400 ml of Methanol Alcohol Solvent in a 1000 ml Flask, and evaporation by rotary evaporation 40C° for drying, and storage at 4C° until use, indicate results that Percentage yield of Alcohol and volatile oils extracted was (3.8% and 4.1%), showing alcohol extract that high positive reaction with used reagent, Alkaloid, Phenol, Glycoside, and Flavonoid, Saponin and tannin. While volatile oil extract revealed positive results for Alkaloid, Glycoside, Flavonoid, tannin and Phenol while and Saponin indicate negative results. The antifungal effects of alcohol extract, volatile oil and alkaloid depended on different concentration, The alcohol extract’s inhibition zone measured 11.12 mm and had a 20 mg/ml concentration, while concentration 40 mg/ml was inhibition zone 12.34mm while concentration 80 mg/ml was inhibition zone 13.65mm, while concentration 100 mg/ml was inhibition zone 15.76 mm, while The inhibition zone of volatile oil extract was 12.76 mm in concentration 0.5 mg/ml, while concentration 0.1 mg/ml was inhibition zone 14.75mm, while concentration 1 mg/ml was inhibition zone 15.76, while concentration 2 mg/ml was inhibition zone 18.65 mm, Alkaloid’s inhibition zone measured 12.34 mm at concentrations of 1 mg/ml and 14.13 mm in those of 5 mg/ml. while concentration 10 mg/ml was inhibition zone 15.98mm, while concentration 15 mg/ml was inhibition zone 20.12 mm. The aim from this study is to determine the best extract (alcohol extract, volatile oil and alkaloid ) isolated from Capsicum frutescens L. fruits on Candida albican.