Leukocytes differential under effect of ciprofloxacin in experimental colibacillosis in chickens

Abstract

Abstract This study was aimed at evaluation of white blood cells of domestic birds over time using a broad-spectrum chemotherapeutic drug Ciprofloxacin. Ciprofloxacin more actively inhibits gram-negative bacteria. In order to evaluate the dynamics, three groups of chickens were formed (1st group – control; 2nd and 3rd groups – experimental ones), which consisted of Hisex Brown cross-bred cockerels. Within the framework of this experiment chickens in the 2nd and 3rd groups were contaminated with Escherichia coli. A day before and for four days after the contamination chickens in the 3rd group received 200 mg/L of ciprofloxacin with water. Blood samples were drawn from all experimental chickens at Day 1, 5, 10, 15 and 20 after the contamination. White blood cell differential count was performed. Analysis of the data obtained showed significant changes in some blood cell parameters in the 2nd and 3rd groups in comparison to the control group. Inflammatory response was detected, which was characterized by significant increase in absolute count and percentage of pseudoeosinophils, monocytes and basophils in the 2nd and 3rd group in comparison to the control group. Nevertheless, white blood cells differential count in the 3rd group was relatively closer to analogous count in the control group.