Optimization of the conditions of rhizogenesis and assessment of the enzymatic activity of the substrates used for rooting of cuttings of berry shrubs in Siberia

Abstract

Abstract Meeting the needs of agricultural production in seedlings of berry crops (black currant (black currant (Ribes nigrum L.), sea buckthorn (Hippophaë rhamnoides L.), edible honeysuckle (Lonicera edulis L)) propagated by green cuttings, requires a search for ways to increase the rooting rate of cuttings Substrates including peat, sand, sapropel with additives of ammonium nitrate were studied. Adding sapropel (pH of water 7.4) to the composition of peat (pH of water 3.0-4.1) + sand increased the rooting rate of green cuttings of berry bushes. Maximum rhizogenesis was observed in the peat + sand + sapropel variant of 20 t/ha. Anthropogenic loads change the biological parameters of substrates and soils. The results of assessing the biological state of substrates used to obtain cuttings of berry bushes in Siberia by the level of enzymatic activity are analyzed. The quality of substrates is analyzed by studies of oxidative and hydrolytic enzymes. It was found that in the options peat + sand + sapropel 20 t/ha and peat + sand + N30, peat + sand + sapropel 10 t/ha + N30 an increase in the activity indicators of urease and invertase enzymes begins. A stable level of activity of the catalase enzyme confirms the bioecological characteristic of the substrate that is optimal for rooting cuttings in the ratio of peat + sand + sapropel 20 t/ha.