Viability and abnormality of sexed spermatozoa with albumin gradient in different diluents and antioxidants treatment

Abstract

Abstract Livestock is an agricultural sub-sector that contributes sufficiently to the needs of protein. One of the bio reproduction technologies to support livestock productivity is sexing technology. The purpose of this study was to measure the viability and abnormality of sexed spermatozoa from sexing with albumin with the treatment of diluents and antioxidants. The material used in this study was fresh semen of 5 PO bulls with progressive motility >70%. The sexing methodology used is 5%, 10%, and 15% albumin (egg white) gradients. The diluent treatment used was CEP-2 and andromed, with the addition or without the 1mM antioxidant (glutation). The sexed semen was made in liquid semen and stored at 3-5 ° C. Sample preparation was done by making a smear of spermatozoa on glass objects and colored with eosin negrosin. Viability and abnormality analysis was performed on days 0 (H0) and 5 (H5). Viability and abnormality analysis of spermatozoa using SCA v.2.1. Parameters measured included: spermatozoa viability and abnormality. The design of the experiment used a 2×2 factorial pattern. Data analyzed with SPSS 24. The viability of spermatozoa from sexing using egg white albumin in andromed diluents is better than CEP-2 on storage days 0-2. Abnormalities of spermatozoa of sexing during cold storage were not influenced by diluents (CEP-2 and andromed) or the use of glutathione. The viability of spermatozoa during cold storage (>80%) and abnormalities (<20%) indicate the quality of liquid semen results from sex is still good.