Characteristics of cytochrome C oxidase subunit I gene in giant clam from Wakatobi National Park Waters, Indonesia

Abstract

Abstract Characteristic of cytochrome C oxidase subunit I (COI) gene in giant clam from Wakatobi National Park Waters was studied to detect the specific nucleotide and show the relationship among species of giant clams. Sample collection was conducted around Wangi-wangi, Kaledupa, and Tomia waters. Genomic DNA was extracted from clam mantle tissue of two generas, and amplified by PCR method. We obtained nucleotide size of 522 bp for genus Tridacna (T. crocea, T. maxima) and 708 bp for genus Hippopus (H. hippopus). There were 52 specific nucleotide for the clams (included T. gigas and T. squamosa from GenBank data). T. crocea, T. gigas, T. squamosa, T. maxima, and H. hippopus had 2, 3, 9, 2, and 36 sites, respectively. Reconstruction phylogenetic tree using Neighbour Joining of 476 bp COI gene sequences showed that all species of giant clams were monophyletic.