The Potential of Oleaginous Filamentous Fungi Isolated from Soil of Baturraden Botanical Garden, Central Java, Indonesia

Abstract

Abstract The present paper introduces potential oleaginous filamentous fungi that have been isolated from the soils of Baturraden Botanical Garden, Central Java, Indonesia. Several types of filamentous fungi were isolated randomly from Baturraden Botanical Garden soils and were screened for their lipid production. Quantitative screening was done using semisynthetic mediums that contain glucose. The potential lipid producers were identified molecularly. The fatty acid profile of three isolates with high lipid content were identified using GC-MS. In the present study, four of nineteen screened fungi accumulated lipid more than 20% of dry cell mass. The lipid content found in BR.2.2, BR.2.3, BR.3.3 and BR.4.4 were 28.44%, 21.06%, 21.76% and 28.27%, respectively. Further analysis showed that three isolates with high lipid content were identified as unclassified strain (BR.2.2), Brevistachys sp. (BR.3.3) and Cerrena sp. (BR.4.4) based on ITS sequence. Isolate BR.2.2 contains 60.47% saturated fatty acid (SFA), 21.12% monounsaturated fatty acid (MUFA) and 5.0% polyunsaturated fatty acid (PUFA). Isolate BR.3.3 contains 36.85% SFA, 17.47% MUFA and 1.59% PUFA. Isolate BR.4.4 contains 70.48% SFA, 19.62% MUFA and 0.56% PUFA. It concludes that lipid produced from isolates BR.2.2, BR.3.3 and BR.4.4 are suitable and have potential for biodiesel production. Further studies to optimize the lipid production are suggested.