A study of the Effect of the Interaction Between the Secretions of the Roots of some Plants of the Cruciferous Family and the Biocontrol Factor Trichoderma spp. in Controlling fusarium wilt Disease on Okra Plants

Abstract

Abstract This study surveyed, identified, and biologically controlled fusarium wilt on okra plants. The field survey found fusarium wilt on okra farms in Najaf, Diwaniyah, and Babylon. 18-73% were infected. Six Fusarium spp. isolates were found in wilted plants. Besides spore shapes and colours, the separated fungus differed in culture colour and growth rate. Fusarium spp. isolates also have different okra plant pathogenicities. However, isolate F2 (Abbasiya isolate) was the most harmful and adopted in following trials as the most virulent. The example isolate was PCR-diagnosed using rDNA-ITS marker. Fusarium solani. Thus, GenBank registered OQ729824. The effect of evaporated and non-evaporated compounds secreted from the tissues of cruciferous plants (Cabbage, broccoli, and cauliflower) on the culture medium P.S.A 1/4 showed that the compounds secreted from the roots of cabbage and broccoli inhibit and prevent the diagonal growth of the pathogenic fungus F. solani at an average rate of 0.00 cm compared to other treatments. Trichoderma spp. biological isolates tolerate cruciferous plant tissues better. The field experiment to assess the efficiency of the interaction between the root secretions of cruciferous family plants (Cabbage, broccoli and cauliflower) and okra plants in the presence of the bioagent factor T. longibrachiatum T2 showed a significant increase in okra plant growth and production. The interaction treatments between the roots of cabbage or broccoli plants in soil contaminated with the pathogenic fungus F. solani, with or without the biological fungus T2, completely protected the okra plants from disease during growth and harvest. Control treatment had 83.33% infection.