Biological evaluation of aqueous extract of oak bark on in vitro models

Abstract

Abstract The article presents the results of studies on the biological activity and effectiveness of drug administration of oak bark extract in various concentrations and mixed with native cicatricial fluid on the luminescence dynamics of a lux biosensor - a genetic engineering strain of Echerichia coli K12 TG1, a constitutively expressing luxCDABE genes of a natural marine microorganism Photobacterium leiongnathi 54D10. It has been established that the administration of oak bark extract, in ratios: 1:12, 1:10, 1:8, (dry matter of bark to distilled water), does not have a toxic effect on the culture of E. coli K12 TG1. The dynamics of feed digestibility after three and six hours of exposure when making oak bark extract in different dosages is accompanied by an increase in the total digestibility coefficient, after 3 hours by a value from 3.92% (P≤0.001) to 21.18% (P≤0.001), after 6 hours the digestibility increases 3.18% (P≤0.001) to 24.25% (P≤0.001) relative to the control. The greatest digestibility is achieved in a dose of 150 μl. Also, a relationship was found between the dose of oak bark extract and the percentage of digestibility: the higher the dosage, the lower the digestibility, and vice versa - the higher the dosage, the lower the coefficient of digestibility of the dry matter of the feed substrate.