Low-cost of shoot propagation of pineapple (Ananas comosus l. Merr) cv Queen using gandasil-D and AB-mix

Abstract

Abstract Media composition is the main factor in the success of plant tissue culture. This study aims to find alternative media formulas using AB-Mix and Gandasil-D for shoot propagation of pineapple. The study used a factorial completely randomized design (CRD). The first factor was the AB-Mix concentration consisting of: 2.5 ml/L, 5.0 ml/L, and 7.5 ml/L. The second factor was the concentration of Gandasil-D consisting of 1.0 g/l, 1.5 g/l, and 2.0 g/l. Each treatment medium added 2 ppm BAP and 0.5 ppm NAA as plant regulators. Parameters observed included the time of shoot emergence, the number of shoots, the number of roots, and leaves number. The results showed that the concentration of AB-Mix affected all observed parameters, while Gandasil-D and the interaction between Gandasil-D and AB-Mix had no significance. AB-Mix 7.5 ml/l was the best treatment because it resulted in shoot emergence time (30.55 days after induction), number of shoots (3.55 shoots/explant), number of roots (2.05 roots/explant), and number of leaves (14.66 leaves/explant) were not significantly from MS media. Based on the regression analysis carried out, it is still possible to increase the concentration of AB-Mix to increase the number of shoots produced.