Antibacterial activity of betel leaf (Piper betle L.) leaves extract on Vibrio harveyi

Abstract

Abstract The use of herbs as a natural medicine for disease control in aquaculture is safer than using antibiotics. Antibiotic resistance from microorganisms is a problem that can impact public health. The purpose of this study was to determine the antibacterial ability of betel leaf (Piper betle L.) toward the pathogenic bacteria, Vibrio harveyi by in vitro. The research design used a completely randomized design. The number of treatments was 12 concentrations of betel leaf extract with three replications for each treatment. The parameters observed were Minimum Inhibition Concentration (MIC) and Minimum Bactericidal Concentration (MBC) from the ethanol extract of betel leaf. The OD value data of the MIC test results were analyzed by Analysis of Variance (ANOVA) and Duncan’s Multiple Range Test. The results proved that betel leaf extract has the potential to be antibacterial with the ability to inhibit the growth of V. harveyi at a minimum concentration of 0.19% (OD: 2.057) while at a minimum concentration of 0.39% it can kill it.