In-Silico Primer Designing and PCR for Detection of Foot and Mouth Disease Virus (FMDV)

Abstract

Abstract This study focuses on Foot-and-mouth disease (FMD), a contagious viral illness affecting cloven-hoofed animals. Caused by the Foot-and-mouth disease virus (FMDV), it is known for rapid replication and various transmission modes. Clinical symptoms vary among animals, including vesicular lesions and lameness in cattle. Global outbreaks continue to pose challenges. Amid the pandemic crisis, quick and sensitive diagnostic tests are essential. Polymerase Chain Reaction (PCR) and Real-time PCR (qPCR) are precise methods for viral detection. The study aims to design FMDV-specific primers using bioinformatics tools, targeting conserved genome regions. The study successfully designs primers, demonstrating specificity against FMDV. These primers hold promise for commercial PCR-based kits, offering rapid, sensitive, and specific FMDV detection in various samples. Further validation on infected cattle is required. In conclusion, this study presents potential primer sets for FMDV detection, contributing to accurate and efficient diagnosis in disease control efforts.