Apoptotic and necrotic lymphocytes after treatment of stem bark extract of Plumeria rubra L invitro

Abstract

Abstract Based on its LC50, stem bark extract of Plumeria rubra L was not toxic to lymphocytes. The goals of this experiment were to investigate the effect of stem bark extract of P. rubra L doses on apoptotic and necrotic lymphocytes. The cells were isolated from peripheral blood using density gradient configuration method to PBMCs. Then, they were cultured in well plates and treated with cisplatin and the stem bark ethanolic extract of P. rubra L in 0, 20, 130, 240, 350, 460, and 570 µg/mL. After 24 hours incubation, the lymphocytes in each sample were stained using annexin V and propidium iodide reagent and ran in a flow cytometer. The data gained were analysed statistically using Kolmogorov Smirnov, one way ANOVA, Lavene’s and LSD tests. The results showed that there were significant apoptotic cell percentage differences (p<0.05) among treatments (p<0.05), with the lowest of 2.22 ± 0.25% and the highest of 14.36 ± 1.06%. However, the necrosis’ was no different, with the lowest of 0.52 ± 0.18% and the highest of 1.85 ± 0.58%. In conclusion, the extract affect the percentage of apoptotic lymphocytes, on the contrary, did not influence the necrotic cells’.