Combination of Moringa oleifera Extract and Fish Albumin can Reduce Inflammatory Cytokine TNFα and IFNγ and Lipid Retention in Steatohepatitis Mice Model

Abstract

Abstract Steatohepatitis is condition where there is accumulation of lipids in the liver. This condition can caused by alcohol consumtion and chemical subtances of industries. Morringa oleifera is a miracle plant that is capable as hepatoprotective and albumin encourages the process of homeostasis in the body. The aim of this study is to determine the effect of M. oleifera and albumin on pro-inflammatory cytokine TNFα and IFNγ and lipid retention on steatohepatitis mice model. The research was conducted using 5 different groups. Normal control was completely untreated. Steatohepatitis control was induced with CCL4 and alcohol without M. oleifera and albumin administration. Three different doses of M.oleifera is used in this study with dose 1000mg/kg BW and albumin was 5300 mg/kg BW. The first dose is combination of M. oleifera 75% and albumin 25%, next dose is 50% M. oleifera and 50% albumin, and the last dose is 25% M. oleifera and 75% albumin. After 2 weeks treatment with M. oleifera and albumin (except normal and steatohepatitis group), mice were sacrificed and the liver and spleen were isolated. The liver tissues were fixed and embeded in parafin to analyse the histopathology by hematoxylin and eosin staining. The spleen used to isolate CD4 T cells and analyse pro-inflammatory cytokine TNFα and IFNγ by flow cytometry analysis. The results showed the reduction of pro-inflammatory cytokine TNFα and IFNγ. Its reduction was linked to the reduction of lipid retention and it shows a promising effect of combination M. oleifera and albumin to treat steatohepatitis.