Goat oocytes quality after vitrification using difference of glycerol concentration and exposure time

Abstract

Abstract The aim of the study was to analyze the effect of glycerol intracellular cryoprotectant concentration and the length of exposure to the morphology of oocytes after cryopreservation using the vitrification method. The research material was Mt-II goat oocytes which had been carried out in IVM media in 24 hours. The study design employed a random design of factorial patterns of 6 x 3, the first factor was glycerol concentration 0, 15, 20, 25, 30, and 35% and the second factor was the exposure time of 1, 3, 5 minutes respectively. Data analyses using analysis of variance. Oocyte morphology is classified as normal if it shows a round shape with intact plasma membrane and round cytoplasm with homogeneous compact granulation, while the morphology is abnormal if it shows a non-spherical shape, zone of the pellucida fracture, the cytoplasm is not spherical and degeneration. The analysis of variance and Duncan Multiple Range Test were employed to analyze the data. The results showed glycerol concentration and exposure time had an impact (P <0.05) on oocyte morphology. The highest proportion of normal oocyte morphology resulted from vitrification using 25% glycerol concentration and 3-minutes exposure time.