Engineering culture using natural filter differences based on microsatelite to improve the quality of Snakehead (Channa striata)

Abstract

Abstract The aim of the study was to examine various biological filters for hyacinth, hydrilla, apu apu and azzola media based microsatellite analysis of quality snakeheads (C striata) production. The research was carried out at the integrated laboratory of Diponegoro University in January to April 2019. The snakeheads used was size 4.83 ± 0.02 cm long of body and weighed 5.47 ± 0.12 g / fish, using artificial feed 35% protein content is given 3% perbiomas per day. The design used CRD 4 treatments and 3 replications were T1 (hyacinth), T2 (Hydrilla), T3 (apu apu), and T4 (Azzola) treatments, then for determining the quality the microsatellite genetic marker IS-GB1F 5-CCC TGT ATT TCA TTT CTC CA -3 and IS-GB 1 R 5-ACC ACT AAC GCA ATC TCT CT-3, for measuring genetic variation, polymorphic, number allel. Data analysis used minitab version 11. The results showed that the best treatment was T3 with survival rate 96.67 ± 1.92%, absolute weight growth of 14.60 ± 2.41g, SGR 2.61 ± 0.22% / day. The quality of snakehead using microsatellite was T3(band10-12, 13-16) on the best quality of snakeheads fish on T3 (204 bps allel ladder with 215 bps.