Comparison of two microbial oil extraction technologies

Abstract

Abstract Microbial oil production has many advantages such as rapid proliferation of microbial cells and short production cycle. The raw materials needed for growth are abundant and inexpensive such as starch, sugar, especially the waste which can be utilized in food or paper industry. 170 oil-producing strains were screened by Sudan black staining observation using fat grains as screening index, among which, 6 high lipid yield yeast strains were identified by residual sugar as indirect index. By comparing the oil-producing ability of this strain, it was determined that the first strain was a good strain for oil production by fermentation. The biomass, oil content and oil yield of the strain were 11.58 g/L, 36.68%, 4.25 g/L, respectively. Through analysis on 26S rDNA sequence and observation on morphological characteristics, culture characteristics and physiological and biochemical characteristics, this strain belonged to Cryptococcus spp.