Improvement of the Ethanol activity by using leaves extract of Artemisia herb alba against Pseudomonas aeuroginosa

Abstract

Abstract The current study objective to improve the influence of ethanol as antibacterial via mixing it with Artemisia herba-alba leaf extract by using a good diffusion method. Ethanol is well known as antimicrobial so that has been used as a hand sanitizer and disinfectant for a long time. Nowadays microbial resistance to disinfectants become a major problem in health care centers, Pseudomonas aeruginosa one of these microbes that tend to create biofilm in low concentrations of ethanol and considered a healthcare-associated pathogen causing nosocomial infections. Seven concentrations of ethanol 70, 60, 50, 40, 30, 20 and 10% were used and mixed with Artemisia herba-alba leaf extract at 50, 25, 12.5, 6.25, 3.12, 1.56 and 0.78 mg/ml in all concentration of ethanol. The results showed that the clear zone (C.Z.) of ethanol alone was 10, 10, 8 and 7 mm by using 70, 60, 50 and 40% respectively, well the low concentrations don't show any antibacterial effect. Additionally, the antibacterial of A. Herba-alba extracts also calculated, showing high C.Z. was 33, 29 and 27 mm at 50, 25 and 12.5 mg/ml, respectively. All the doses of mixture ethanol and A. Herba-alba extract showed a higher effect comparing to previous results, where 50 mg extract mixed with 70% and 60% ethanol showed C.Z. 39 mm also the low concentration of ethanol showed antibacterial effect up to 33 mm, Even 0.78 mg of extract show inducing in the effect of ethanol against P. Aeruginosa. In conclusion, mixing A. Herba-alba leaf extract can improve the antibacterial effect of ethanol.