Activation of Artemia cysts with the use of different substances

Abstract

Abstract The aim of the study was to study the use of hydrogen peroxide and ascorbic acid of different concentrations as an activator for the incubation of artemia cysts. The work was carried out on cysts of artemia taken from several lakes of the Novosibirsk region (Western Siberia). During incubation, a standard method was used: the salinity of the water was 3%, the water temperature was 28□C, continuous aeration was carried out, constant illumination was 2000 lux. An activator was added to the water at different concentrations: ascorbic acid - 0.05, 0.1, 0.5, 1.0 g/l; hydrogen peroxide - 0.4; 0.6; 0.8; 1. The maximum hatching was observed at a concentration of 0.6 g/l of hydrogen peroxide, 82% for cysts from Lake Sakhalin and 68% - from Lake Barbashi. When using ascorbic acid, the maximum hatching of cysts was observed at a concentration of 0.1 g/l: Sakhalin - 81.4, Pokrovka - 75.3 and Barbashi - 66.7%. When using other concentrations of activators, the percentage of cysts hatching decreased. The obtained results indicate the need to select the activating substance and its concentration of activation of cysts for each lake individually.