Screening of Anti-MRSA metabolites in bacteria symbiotic with Batissa violaceae celebensis marten 1897

Abstract

Abstract Currently, infectious diseases are still a serious problem in Indonesia, especially with thewidespread resistance of microbes to antibiotics. Staphylococcus aureus as one of the commonpathogens causing infection has experienced resistance to various classes of beta-lactam antibiotics known as Methicillin-resistant Staphylococcus aureus (MRSA). Therefore antibiotic alternatives sourced from nature are needed to treat the MRSA pathogenic infection. This study aims to explore the potential of bacteria in symbiosis with Pokea Shells (Batissaviolaceacelebensis Martens 1897) from Konawe Regency, Southeast Sulawesi Province to be used as the newest anti-MRSA. In this research, Pokea Shell’s symbiont bacteria were isolated from Konawe Regency to obtain pure isolates. Pure isolates that have been produced from secondary metabolites to be tested for their potential in inhibiting the growth of pathogenic bacteria MRSA. The inhibition test was done qualitatively by using a paper disk. Based on the results five bacterial isolates were obtained with different abilities to inhibit MRSA bacteria. Based on the qualitative test obtained that the Bvc1 and Bvc3 bacterial isolates had the potency to be used as anti-MRSA agents. These isolates have an inhibition zone of 25 mm and 22 mm with a sensitive category according to CLSI standards (Sensitive ≥17 mm). Determination of the gram of these 2 isolates found as gram-positive bacteria in the form of bacilli so it can be concluded that the 2 isolates can be used as anti-MRSA agents.