Fractionation of secondary metabolites from Serratia plymuthica UBCF_13 based on polarity properties

Abstract

Abstract Serratia plymuthica UBCF_13 bacteria produce compounds capable of suppressing the growth of various pathogenic fungi. There are several types of compounds produced by these bacteria. The compounds produced by S. plymuthica bacteria have different abilities and characteristics. So, to determine the capabilities and characteristics of each compound, a compound separation technique or method is needed. This study was conducted to determine the optimal mobile phase composition for the separation of compounds. Optimization of the mobile phase was carried out by spotting the extracellular extract of S. plymuthica on thin layer chromatography (TLC) using several comparisons of ethyl acetate with hexane and ethyl acetate with methanol. The separation technique in this research will be useful for further study of the antifungal compound Serratia plymuthica UBCF_13, such as the identification and characterization of each of the compounds it produces. In this study, the best mobile phase was not found in the separation of compounds. There are several mobile phases that separate the compounds, for further test the elution gradient method is used.