The Decreasing of Quality Liquid Semen Using Four Months Storages of Tris Aminomethan and CEP-3 Diluents

Abstract

Abstract The purpose of this research was to evaluate the decreasing of Limmousin liquid semen quality using four months storages of Tris Aminomethan and Caudal Epididymal Plasma (CEP-3) diluents. This research was conducted at Animal Reproduction Laboratory, Faculty of Animal Science, University of Brawijaya. The material used was fresh semen of Limousin bull which was collected in artificial insemination center Singosari. The method used was experimental laboratory with two treatments and 10 replications including T0 (Tris Aminomethan + 20% Egg Yolk) and T1 (CEP-3 + 10% Egg Yolk). Data was analyzed by using t-paired test. The result showed that Tris Aminomethan + 20% Egg Yolk gave the best result in percentage of motility, viability and abnormality. The individu motility of T0 was higher (27.3±1.4) than T1 (26.8±2.4). The percentage of viability on T0 was higher (60.5±1.1) than T1 (60.0±3.0). while the percentage of abnormality was lower (13.9±0.4) than T1 (14.4±2.6). In conclusion after 4 months storage of diluents, tris Aminomethan + 20% egg yolk gave the best treatment in motility, viability and abnormality in liquid semen.