Biotechnological Techniques to Improve Regenerative Abilities of Liver Tissue in Experimental Cholestasis

Abstract

Abstract The study examined the effect of plant hepatoprotectors obtained in the process of biotechnological techniques and thermal effects on liver tissue to prevent the destruction of cell membranes and stimulate regenerative processes in hepatocytes in experimental models (12 dogs). All animals underwent complex conservative therapy, including a plant hepatoprotector with the code name GTPS-4, containing a purified dry extract from the fruits of milk thistle, dry extract from tansy, St. John’s wort, turmeric, birch leaves and immortelle flowers. Conservative therapy was combined with thermal effects on the liver tissue to enhance reparative regeneration. The combination of herbal preparations and contact coagulation techniques gave the best result, and the optimal combination of herbal preparations in the form of collection was also proposed. The structural and functional state of the liver was assessed according to the results of morphological studies. The performed complex therapy significantly improves the condition of the liver tissues, its regenerative abilities and normalizes homeostasis indicators. The inclusion of plant hepatoprotectors in complex conservative therapy is a promising direction in the development of biotechnology in hepatology.