The effectiveness of yam (Pachyrhizus erosus) juice in maintaining the viability of Trichoderma viride spores

Abstract

Abstract Trichoderma viride is a potential biological agent to control main estate crop diseases. One of the disadvantages of this fungus is susceptible to ultraviolet (UV) from sunlight. This study aimed to evaluate the effectiveness of yam juice (YJ) in protecting T. viride spores against UV exposure. The research was conducted from March to July 2019 at the Plant Protection Laboratory of IIBCRI, Sukabumi. The suitability as a growth medium was performed by incorporating 0%, 1%, 5% 10%, and 20% concentration of YJ into potato dextrose agar (PDA) medium. The spore viability test used a 20% concentration of YJ in the talc carrier medium compared to control (without YJ) and exposing treatment for 0, 60, 120, and 180 minutes. The results showed that YJ was suitable for T. viride. The original viability remaining (OVR) were 77.9%, 32.62%, and 13.67% after exposing 60, 120, and 180 minutes under sunlight, and 40.01%, 35.78%, and 32.62% respectively under UV lamp. The relative efficiency (RE) value of YJ treatment after exposure 60, 120, and 180 minutes under sunlight were 4.72, 3.52, and 4.42 respectively, and under UV lamp were 1.69, 1.65, and 1.73 respectively. YJ effectively protects T. viride spores from UV exposure.