The phytonematodes species on sugarcane plants (Saccharum officinarum L.) at Subang Sugar Factory PT PG Rajawali II, West Java

Abstract

Abstract Phytonematodes are an important plant pest in sugarcane. The presence of phytonematodes can cause root damage and eventually reduce plant productivity. This study aimed to identify and calculated the abundance of sugarcane phytonematodes species in the Subang Sugar Factory of PT PG Rajawali II, West Java. The symptoms of sugarcane plants infected by phytonematodes include stunting plants and slender stems, and necrotic roots with blackish purple spots that spread unevenly on young roots. The diagnostic samples, consisted of roots and soil, were taken from the second ratoon cane. The stages of the research included nematode extraction from soil sample by centrifugation flotation method and extraction nematode from the root using the mist chamber method, nematode staining inside root tissue, and nematode identification. The nematode species were identified based on the morphological characters. Eight phytonematodes species, namely Pratylenchus zeae, Xiphinema setariae, Hoplolaimus indicus, Rotylenchulus reniformis, Criconemoides morgenensis, Coslenchus paramaritus, Helicotylenchus sp., Coslenchus paramaritus and Tylenchus sp. were identified. Based on the highest absolute population in sugarcane roots was Pratylenchus zeae.