Biosynthesized TiO2 nanoparticles and their applications for the treatment of pediatric acute leukemia

Abstract

Abstract The current study demonstrates the efficiency of Salvia Spinosa (S. Spinosa) leaf extract in the biofabrication of Titanium dioxide nanoparticles (TiO2 NPs). An easy and green method was used to synthesize TiO2 NPs, which involves the addition of Titanium hydroxide [Ti(OH)2] solution and the leaf extract of S. Spinosa, followed by stirring results in the formation of a light green dispersion of TiO2 NPs. Characterization of the formed TiO2 NPs was performed using the spectroscopic techniques such as FTIR, XRD, EDS, SAED and TEM. The formation of polydisperse TiO2 NPs with a mean particle size of 23 nm was revealed by TEM analysis. The biomolecules that are possibly involved in reducing TiO2 ions are shown in the FTIR spectrum of the S. Spinosa plant leaf extract. Zeta potential results have confirmed the extreme negative charge of 19 mV for the formed TiO2 NPs. The crystalline structure of the fabricated NPs was revealed by the analysis of XRD. In addition, the cytotoxicity results have confirmed that the cytotoxicity against acute lymphoblastic leukemia (Molt-4) cells depends on the dose, further signifying their potential for the advance of new materials and techniques for pediatric leukemia treatment.