Study on adsorption behaviours of bovine serum albumin on CoCrMo alloy and Al2O3 ceramic surfaces

Abstract

Abstract The adsorption behaviours of bovine serum albumin (BSA) molecules on CoCrMo alloy and Al2O3 ceramic were considered as the main research object in this study, and atomic force microscopy (AFM) was used for investigation. The thickness of the BSA films as well as adsorption forces between the BSA molecules and samples were tested. The results indicate that the thickness of the adsorbed film is close to 2.09 nm, exhibiting lateral monolayer adsorption as the main mode of adsorption of deformed BSA molecules on the material surfaces; the adsorption force of the BSA molecules on CoCrMo alloy is greater than 85.9 nN, and the adsorption force on Al2O3 ceramic is greater than 68.2 nN. This difference in the adsorption force may be related to the fact that the surface positive charge of the CoCrMo alloy is greater than that of the Al2O3 ceramic.