Green synthesis of ZnONps using Horse gram seed aqueous extract and its in vitro evaluation on antioxidant, antidiabetic, anticancer and DNA binding potential

Abstract

Abstract The goal of this study was to access the green production of zinc oxide nanoparticles (G-ZnO NPs) using aqueous extract of horse gram seeds (Macrotyloma Uniflorum). The precursor to the extract ratio (2.5:1) and pH value (8.5), along with the zinc nitrate concentration (0.5 M), had an impact on the particle size and the green synthesized ZnO nanoparticles. UV Spectroscopy inspection revealed formation of G-ZnO NPs with absorption at 320 nm which is the characteristic absorption of G-ZnO NPs. FTIR, XRD, SEM, EDX and TEM were used to characterize the green synthesized ZnO NPs. The findings demonstrated that the presence of secondary metabolites in the seed extractstabilize and contribute in the production of G-ZnO NPs. A dose-dependent increase in antibacterial activity was seen in evaluation of these NPs against Klebsiella, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa. The antioxidant activity and antidiabetic activity for G-ZnO NPs was also noted to be concentration dependent. The synthesized nanoparticles are found to interact with CT-DNA to produce a hypochromic shift. Further the studies on the G-ZnONPs in MCF-7 cells using the MTT test demonstrated greater cellular inhibition. The results validate that the green synthesized ZnO-NPs from horse gram seed possess good biological activities and can be an excellent biologically derived potential material which could be used in the drug discovery from natural products.