Abstract
C/D box small nucleolar RNAs (SNORDs) are small noncoding RNAs, and their best-understood function is to target the methyltransferase fibrillarin to rRNA (for example,SNORD27performs 2′-O-methylation of A27 in 18S rRNA). Unexpectedly, we found a subset of SNORDs, includingSNORD27, in soluble nuclear extract made under native conditions, where fibrillarin was not detected, indicating that a fraction of theSNORD27RNA likely forms a protein complex different from canonical snoRNAs found in the insoluble nuclear fraction. As part of this previously unidentified complex,SNORD27regulates the alternative splicing of the transcription factorE2F7pre-mRNA through direct RNA–RNA interaction without methylating the RNA, likely by competing withU1small nuclear ribonucleoprotein (snRNP). Furthermore, knockdown ofSNORD27activates previously “silent” exons in several other genes through base complementarity across the entireSNORD27sequence, not just the antisense boxes. Thus, some SNORDs likely function in both rRNA and pre-mRNA processing, which increases the repertoire of splicing regulators and links both processes.
Funder
American Heart Association
Publisher
Proceedings of the National Academy of Sciences
Cited by
149 articles.
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