De novo design of symmetric ferredoxins that shuttle electrons in vivo

Author:

Mutter Andrew C.,Tyryshkin Alexei M.,Campbell Ian J.,Poudel Saroj,Bennett George N.,Silberg Jonathan J.,Nanda VikasORCID,Falkowski Paul G.

Abstract

A symmetric origin for bacterial ferredoxins was first proposed over 50 y ago, yet, to date, no functional symmetric molecule has been constructed. It is hypothesized that extant proteins have drifted from their symmetric roots via gene duplication followed by mutations. Phylogenetic analyses of extant ferredoxins support the independent evolution of N- and C-terminal sequences, thereby allowing consensus-based design of symmetric 4Fe-4S molecules. All designs bind two [4Fe-4S] clusters and exhibit strongly reducing midpoint potentials ranging from −405 to −515 mV. One of these constructs efficiently shuttles electrons through a designed metabolic pathway inEscherichia coli. These finding establish that ferredoxins consisting of a symmetric core can be used as a platform to design novel electron transfer carriers for in vivo applications. Outer-shell asymmetry increases sequence space without compromising electron transfer functionality.

Funder

Gordon and Betty Moore Foundation

U.S. Department of Energy

NASA | Ames Research Center

Publisher

Proceedings of the National Academy of Sciences

Subject

Multidisciplinary

Reference52 articles.

1. The rise of oxygen in Earth’s early ocean and atmosphere

2. The Bakerian Lecture, 1981 Natural Selection of the Chemical elements

3. Iron-sulfur proteins: ancient structures, still full of surprises

4. R. Cammack , “Iron and sulfur in the origin and evolution of biological energy conversion systems” in Origin and Evolution of Biological Energy Conversion, Ed H. Baltscheffsky (Wiley-VCH, 1996), pp. 43–69.

5. Evolution of the Structure of Ferredoxin Based on Living Relics of Primitive Amino Acid Sequences

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