Author:
Bardy Cedric,van den Hurk Mark,Eames Tameji,Marchand Cynthia,Hernandez Ruben V.,Kellogg Mariko,Gorris Mark,Galet Ben,Palomares Vanessa,Brown Joshua,Bang Anne G.,Mertens Jerome,Böhnke Lena,Boyer Leah,Simon Suzanne,Gage Fred H.
Abstract
Human cell reprogramming technologies offer access to live human neurons from patients and provide a new alternative for modeling neurological disorders in vitro. Neural electrical activity is the essence of nervous system function in vivo. Therefore, we examined neuronal activity in media widely used to culture neurons. We found that classic basal media, as well as serum, impair action potential generation and synaptic communication. To overcome this problem, we designed a new neuronal medium (BrainPhys basal + serum-free supplements) in which we adjusted the concentrations of inorganic salts, neuroactive amino acids, and energetic substrates. We then tested that this medium adequately supports neuronal activity and survival of human neurons in culture. Long-term exposure to this physiological medium also improved the proportion of neurons that were synaptically active. The medium was designed to culture human neurons but also proved adequate for rodent neurons. The improvement in BrainPhys basal medium to support neurophysiological activity is an important step toward reducing the gap between brain physiological conditions in vivo and neuronal models in vitro.
Funder
Marie Curie International Outgoing Fellowship For Career Development
IPSEN PHARMA S.A.S
Annette C. Merle-Smith
The Leona M. and Harry B. Hemsley Charitable Trust
Bob and Mary Jane Engman
JPB Foundation
LGMD2i Research Fund, Erna and Andrew Viterbi and the Viterbi Family of the Jewish Community Foundation San Diego Neuroscience Research Initiative
G Harold and Leila Y. Mathers Foundation
HHS | NIH | National Institute of Mental Health
Publisher
Proceedings of the National Academy of Sciences
Cited by
320 articles.
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