Author:
Mills Jeremy H.,Sheffler William,Ener Maraia E.,Almhjell Patrick J.,Oberdorfer Gustav,Pereira José Henrique,Parmeggiani Fabio,Sankaran Banumathi,Zwart Peter H.,Baker David
Abstract
Metal-chelating heteroaryl small molecules have found widespread use as building blocks for coordination-driven, self-assembling nanostructures. The metal-chelating noncanonical amino acid (2,2′-bipyridin-5yl)alanine (Bpy-ala) could, in principle, be used to nucleate specific metalloprotein assemblies if introduced into proteins such that one assembly had much lower free energy than all alternatives. Here we describe the use of the Rosetta computational methodology to design a self-assembling homotrimeric protein with [Fe(Bpy-ala)3]2+ complexes at the interface between monomers. X-ray crystallographic analysis of the homotrimer showed that the design process had near-atomic-level accuracy: The all-atom rmsd between the design model and crystal structure for the residues at the protein interface is ∼1.4 Å. These results demonstrate that computational protein design together with genetically encoded noncanonical amino acids can be used to drive formation of precisely specified metal-mediated protein assemblies that could find use in a wide range of photophysical applications.
Funder
DOD | ONR | Office of Naval Research Global
| Basic Energy Sciences
HHS | NIH | National Institute of General Medical Sciences
DOD | Defense Threat Reduction Agency
Swiss National Science Foundation
European Commission
Publisher
Proceedings of the National Academy of Sciences
Cited by
38 articles.
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