Author:
Han Byung Hee,Zhou Meng-liang,Johnson Andrew W.,Singh Itender,Liao Fan,Vellimana Ananth K.,Nelson James W.,Milner Eric,Cirrito John R.,Basak Jacob,Yoo Min,Dietrich Hans H.,Holtzman David M.,Zipfel Gregory Joseph
Abstract
Cerebral amyloid angiopathy (CAA) is characterized by deposition of amyloid β peptide (Aβ) within walls of cerebral arteries and is an important cause of intracerebral hemorrhage, ischemic stroke, and cognitive dysfunction in elderly patients with and without Alzheimer’s Disease (AD). NADPH oxidase-derived oxidative stress plays a key role in soluble Aβ-induced vessel dysfunction, but the mechanisms by which insoluble Aβ in the form of CAA causes cerebrovascular (CV) dysfunction are not clear. Here, we demonstrate evidence that reactive oxygen species (ROS) and, in particular, NADPH oxidase-derived ROS are a key mediator of CAA-induced CV deficits. First, the NADPH oxidase inhibitor, apocynin, and the nonspecific ROS scavenger, tempol, are shown to reduce oxidative stress and improve CV reactivity in aged Tg2576 mice. Second, the observed improvement in CV function is attributed both to a reduction in CAA formation and a decrease in CAA-induced vasomotor impairment. Third, anti-ROS therapy attenuates CAA-related microhemorrhage. A potential mechanism by which ROS contribute to CAA pathogenesis is also identified because apocynin substantially reduces expression levels of ApoE—a factor known to promote CAA formation. In total, these data indicate that ROS are a key contributor to CAA formation, CAA-induced vessel dysfunction, and CAA-related microhemorrhage. Thus, ROS and, in particular, NADPH oxidase-derived ROS are a promising therapeutic target for patients with CAA and AD.
Funder
HHS | NIH | National Institute of Neurological Disorders and Stroke
HHS | National Institutes of Health
Publisher
Proceedings of the National Academy of Sciences
Reference72 articles.
1. Sporadic and familial cerebral amyloid angiopathies;Revesz;Brain Pathol,2002
2. Predominant deposition of amyloid-beta 42(43) in plaques in cases of Alzheimer’s disease and hereditary cerebral hemorrhage associated with mutations in the amyloid precursor protein gene;Mann;Am J Pathol,1996
3. Series Introduction: Alzheimer’s disease: perspectives for the new millennium
4. Alzheimer’s disease: Genes, proteins, and therapy;Selkoe;Physiol Rev,2001
5. Clinical phenotypes of Cerebral Amyloid Angiopathy
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