Mixing and matching TREK/TRAAK subunits generate heterodimeric K2P channels with unique properties

Author:

Blin Sandy,Ben Soussia Ismail,Kim Eun-Jin,Brau Frédéric,Kang Dawon,Lesage Florian,Bichet Delphine

Abstract

The tandem of pore domain in a weak inwardly rectifying K+ channel (Twik)-related acid-arachidonic activated K+ channel (TRAAK) and Twik-related K+ channels (TREK) 1 and TREK2 are active as homodimers gated by stretch, fatty acids, pH, and G protein-coupled receptors. These two-pore domain potassium (K2P) channels are broadly expressed in the nervous system where they control excitability. TREK/TRAAK KO mice display altered phenotypes related to nociception, neuroprotection afforded by polyunsaturated fatty acids, learning and memory, mood control, and sensitivity to general anesthetics. These channels have emerged as promising targets for the development of new classes of anesthetics, analgesics, antidepressants, neuroprotective agents, and drugs against addiction. Here, we show that the TREK1, TREK2, and TRAAK subunits assemble and form active heterodimeric channels with electrophysiological, regulatory, and pharmacological properties different from those of homodimeric channels. Heteromerization occurs between all TREK variants produced by alternative splicing and alternative translation initiation. These results unveil a previously unexpected diversity of K2P channels that will be challenging to analyze in vivo, but which opens new perspectives for the development of clinically relevant drugs.

Funder

Fondation pour la Recherche Médicale

Agence Nationale de la Recherche

National Research Foundation of Korea

Publisher

Proceedings of the National Academy of Sciences

Subject

Multidisciplinary

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