CHAF1A/B mediate silencing of unintegrated HIV-1 DNAs early in infection-Reference-Cited by-同舟云学术

CHAF1A/B mediate silencing of unintegrated HIV-1 DNAs early in infection

Published:2022-01-24 Issue:4 Volume:119 Page:
ISSN:0027-8424
Container-title:Proceedings of the National Academy of Sciences
language:en
Short-container-title:Proc. Natl. Acad. Sci. U.S.A.

Author:

Geis Franziska K.¹²³⁴,Sabo Yosef⁴⁵,Chen Xiao⁶⁷^ORCID,Li Yinglu⁶⁷,Lu Chao⁶⁷,Goff Stephen P.¹²³⁴⁷^ORCID

Affiliation:

1. Department of Biochemistry and Molecular Biophysics, Columbia University Medical Center, New York, NY 10032

2. Department of Microbiology and Immunology, Columbia University Medical Center, New York, NY 10032

3. HHMI, Columbia University Medical Center, New York, NY 10032

4. Aaron Diamond AIDS Research Center, Columbia University Medical Center, New York, NY 10032

5. Division of Infectious Diseases, Department of Medicine, Columbia University Medical Center, New York, NY 10032

6. Department of Genetics and Development, Columbia University Medical Center, New York, NY 10032

7. Herbert Irving Comprehensive Cancer Center, Columbia University Medical Center, New York, NY 10032

Abstract

Significance Invading viral DNAs constitute a high risk for the infected cell and are high-profile targets of antiviral host factors. Nevertheless, little is known about the silencing machinery in the nucleus that acts to prevent transcription or retroviral integration of extrachromosomal DNA. We here identified CHAF1A and CHAF1B as two players that mediate silencing of unintegrated HIV-1 DNAs. Our findings provide evidence that these factors act independently of their canonical nucleosome assembly complex to induce silencing early in infection. The characterization of the intrinsic cellular defense mechanism against incoming DNA is relevant to transient gene delivery, as mediated by virus-based vectors in gene therapy.

Funder

HHS | NIH | National Cancer Institute

Howard Hughes Medical Institute

German Research Foundation

HHS | NIH | National Institute of General Medical Sciences

Publisher

Proceedings of the National Academy of Sciences

Subject

Multidisciplinary

Link

https://pnas.org/doi/pdf/10.1073/pnas.2116735119

Reference53 articles.

1. J. M. Coffin, S. H. Hughes, H. E. Varmus, “The interactions of retroviruses and their hosts” in Retroviruses, J. M. Coffin, S. H. Hughes, H. E. Varmus, Eds. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1997).

2. R. Swanstrom, J. W. Wills, “Synthesis, assembly, and processing of viral proteins” in Retroviruses, J. M. Coffin, S. H. Hughes, H. E. Varmus, Eds. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1997).

3. Restriction endonuclease cleavage of linear and closed circular murine leukemia viral DNAS: Discovery of a smaller circular form

4. Roles of host cell factors in circularization of retroviral dna

5. Construction and analysis of deletion mutations in the pol gene of moloney murine leukemia virus: A new viral function required for productive infection

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