Eukaryotic catecholamine hormones influence the chemotactic control of Vibrio campbellii by binding to the coupling protein CheW

Author:

Weigert Muñoz Angela1ORCID,Hoyer Elisabeth2ORCID,Schumacher Kilian2ORCID,Grognot Marianne3ORCID,Taute Katja M.3ORCID,Hacker Stephan M.45ORCID,Sieber Stephan A.16,Jung Kirsten2ORCID

Affiliation:

1. Center for Functional Protein Assemblies, Technical University of Munich, 85748 Garching, Germany

2. Department of Biology I, Microbiology, Ludwig-Maximilians-University München, 82152 Martinsried, Germany

3. Rowland Institute, Harvard University, Cambridge, MA 02142

4. Leiden Institute of Chemistry, Leiden University, 2333 CC Leiden, Netherlands

5. Department of Chemistry, Technical University of Munich, 85748 Garching, Germany

6. Helmholtz Institute for Pharmaceutical Research Saarland, Helmholtz Center for Infection Research, 66123 Saarbrücken, Germany

Abstract

Significance Host-emitted stress hormones significantly influence the growth and behavior of various bacterial species; however, their cellular targets have so far remained elusive. Here, we used customized probes and quantitative proteomics to identify the target of epinephrine and the α-adrenoceptor agonist phenylephrine in live cells of the aquatic pathogen Vibrio campbellii . Consequently, we have discovered the coupling protein CheW, which is in the center of the chemotaxis signaling network, as a target of both molecules. We not only demonstrate direct ligand binding to CheW but also elucidate how this affects chemotactic control. These findings are pivotal for further research on hormone-specific effects on bacterial behavior.

Publisher

Proceedings of the National Academy of Sciences

Subject

Multidisciplinary

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