Protein and lipid mass concentration measurement in tissues by stimulated Raman scattering microscopy-Reference-Cited by-同舟云学术

Protein and lipid mass concentration measurement in tissues by stimulated Raman scattering microscopy

Published:2022-04-22 Issue:17 Volume:119 Page:
ISSN:0027-8424
Container-title:Proceedings of the National Academy of Sciences
language:en
Short-container-title:Proc. Natl. Acad. Sci. U.S.A.

Author:

Oh Seungeun¹^ORCID,Lee ChangHee²,Yang Wenlong³,Li Ang¹,Mukherjee Avik¹,Basan Markus¹,Ran Chongzhao⁴^ORCID,Yin Wei⁴,Tabin Clifford J.²,Fu Dan⁵^ORCID,Xie X. Sunney⁶,Kirschner Marc W.¹^ORCID

Affiliation:

1. Department of Systems Biology, Harvard Medical School, Boston, MA 02115

2. Department of Genetics, Harvard Medical School, Boston, MA 02115

3. Center for Advanced Imaging, Harvard University, Cambridge, MA 20138

4. Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02129

5. Department of Chemistry, University of Washington, Seattle, WA 98195

6. Biomedical Pioneering Innovation Center, Peking University, Beijing 100871; China

Abstract

Significance We report a quantitative Raman microscopy method that measures the concentration of protein and lipid in cells at high spatial resolution in living and in fixed samples of tissues, allowing quantitative studies of cell size and organelle regulation both in cell culture and in tissue slices; it can be applied to problems of cell size control, intracellular crowding, and lipid metabolism in the context of cell growth, cell differentiation, cell senescence, and pathology.

Funder

HHS | NIH | National Institute of General Medical Sciences

HHS | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development

HHS | NIH | National Institute of Biomedical Imaging and Bioengineering

HHS | NIH | National Institute on Aging

Publisher

Proceedings of the National Academy of Sciences

Subject

Multidisciplinary

Link

https://pnas.org/doi/pdf/10.1073/pnas.2117938119

Reference73 articles.