Pseudomonas aeruginosa distinguishes surfaces by stiffness using retraction of type IV pili

Abstract

Significance While many bacteria can sense the presence of a surface, the mechanical properties of different surfaces vary tremendously and can be as rigid as bone or as soft as mucus. We show that the pathogen Pseudomonas aeruginosa distinguishes surfaces by stiffness and transcriptionally tunes its virulence to surface rigidity. This connection between pathogenicity and mechanical properties of the infection site presents an interesting potential for clinical applications. The mechanism behind stiffness sensing relies on the retraction of external appendages called type IV pili that deform the surface. While this mechanism has interesting parallels to stiffness sensing in mammalian cells, our results suggest that stiffness sensing in much smaller bacterial cells relies on temporal sensing instead of spatial sensing strategies.