Conformational alterations in unidirectional ion transport of a light-driven chloride pump revealed using X-ray free electron lasers-Reference-Cited by-同舟云学术

Conformational alterations in unidirectional ion transport of a light-driven chloride pump revealed using X-ray free electron lasers

Published:2022-02-23 Issue:9 Volume:119 Page:
ISSN:0027-8424
Container-title:Proceedings of the National Academy of Sciences
language:en
Short-container-title:Proc. Natl. Acad. Sci. U.S.A.

Author:

Hosaka Toshiaki¹^ORCID,Nomura Takashi²³^ORCID,Kubo Minoru³^ORCID,Nakane Takanori⁴,Fangjia Luo²⁵,Sekine Shun-ichi¹^ORCID,Ito Takuhiro¹^ORCID,Murayama Kazutaka¹⁶^ORCID,Ihara Kentaro¹,Ehara Haruhiko¹^ORCID,Kashiwagi Kazuhiro¹,Katsura Kazushige¹,Akasaka Ryogo¹,Hisano Tamao¹,Tanaka Tomoyuki²⁵,Tanaka Rie²⁵,Arima Toshi²⁵,Yamashita Ayumi²⁵,Sugahara Michihiro²,Naitow Hisashi²,Matsuura Yoshinori²,Yoshizawa Susumu⁷,Tono Kensuke²⁸^ORCID,Owada Shigeki²⁸^ORCID,Nureki Osamu⁴^ORCID,Kimura-Someya Tomomi¹^ORCID,Iwata So²⁵,Nango Eriko²⁹^ORCID,Shirouzu Mikako¹

Affiliation:

1. RIKEN Center for Biosystems Dynamics Research, Yokohama, Kanagawa 230-0045, Japan

2. RIKEN SPring-8 Center, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan

3. Department of Life Science, Graduate School of Science, University of Hyogo, Hyogo 678-1297, Japan

4. Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo 113-0034, Japan

5. Department of Cell Biology, Graduate School of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan

6. Division of Biomedical Measurements and Diagnostics, Graduate School of Biomedical Engineering, Tohoku University, Sendai 980-8575, Japan

7. Atmosphere and Ocean Research Institute, The University of Tokyo, Chiba 277-8564, Japan

8. Japan Synchrotron Radiation Research Institute, Sayo-cho, Sayo-gun, Hyogo 679-5198, Japan

9. Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Aoba-ku, Sendai 980-8577, Japan

Abstract

Significance Light-driven chloride pumps have been identified in various species, including archaea and marine flavobacteria. The function of ion transportation controllable by light is utilized for optogenetics tools in neuroscience. Chloride pumps differ among species, in terms of amino acid homology and structural similarity. Our time-resolved crystallographic studies using X-ray free electron lasers reveal the molecular mechanism of halide ion transfer in a light-driven chloride pump from a marine flavobacterium. Our data indicate a common mechanism in chloride pumping rhodopsins, as compared to previous low-temperature trapping studies of chloride pumps. These findings are significant not only for further improvements of optogenetic tools but also for a general understanding of the ion pumping mechanisms of microbial rhodopsins.

Funder

MEXT | Japan Society for the Promotion of Science

Japan Agency for Medical Research and Development

MEXT | RIKEN

Publisher

Proceedings of the National Academy of Sciences

Subject

Multidisciplinary

Link

https://pnas.org/doi/pdf/10.1073/pnas.2117433119

Reference54 articles.

1. Functions of a New Photoreceptor Membrane