Abstract
Mechanistic understanding of the factors that govern host tropism remains incompletely understood for most pathogens.Brucellaspecies, which are capable of infecting a wide range of hosts, offer a useful avenue to address this question. We hypothesized that metabolic fine-tuning to intrahost niches is likely an underappreciated axis underlying pathogens’ ability to infect new hosts and tropism. In this work, we compared the central metabolism of sevenBrucellaspecies by stable isotopic labeling and genetics. We identified two functionally distinct groups, one overlapping with the classical zoonotic species of domestic livestock that exclusively use the pentose phosphate pathway (PPP) for hexose catabolism, whereas species from the second group use mostly the Entner–Doudoroff pathway (EDP). We demonstrated that the metabolic dichotomy amongBrucellaeemerged after the acquisition of two independent EDP-inactivating mutations in all classical zoonotic species. We then examined the pathogenicity of key metabolic mutants in mice and confirmed that this trait is tied to virulence. Altogether, our data are consistent with the hypothesis that the PPP has been incrementally selected over the EDP in parallel toBrucellaadaptation to domestic livestock.
Publisher
Proceedings of the National Academy of Sciences
Cited by
11 articles.
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