IL-36R signaling integrates innate and adaptive immune-mediated protection against enteropathogenic bacteria

Author:

Ngo Vu L.,Abo Hirohito,Kuczma MichalORCID,Szurek Edyta,Moore Nora,Medina-Contreras Oscar,Nusrat Asma,Merlin Didier,Gewirtz Andrew T.,Ignatowicz LeszekORCID,Denning Timothy L.

Abstract

Enteropathogenic bacterial infections are a global health issue associated with high mortality, particularly in developing countries. Efficient host protection against enteropathogenic bacterial infection is characterized by coordinated responses between immune and nonimmune cells. In response to infection in mice, innate immune cells are activated to produce interleukin (IL)-23 and IL-22, which promote antimicrobial peptide (AMP) production and bacterial clearance. IL-36 cytokines are proinflammatory IL-1 superfamily members, yet their role in enteropathogenic bacterial infection remains poorly defined. Using the enteric mouse pathogen,C.rodentium, we demonstrate that signaling via IL-36 receptor (IL-36R) orchestrates a crucial innate-adaptive immune link to control bacterial infection. IL-36R-deficient mice (Il1rl2−/−) exhibited significant impairment in expression of IL-22 and AMPs, increased intestinal damage, and failed to containC. rodentiumcompared to controls. These defects were associated with failure to induce IL-23 and IL-6, two key IL-22 inducers in the early and late phases of infection, respectively. Treatment ofIl1rl2−/−mice with IL-23 during the early phase ofC. rodentiuminfection rescued IL-22 production from group 3 innate lymphoid cells (ILCs), whereas IL-6 administration during the late phase rescued IL-22-mediated production from CD4+T cell, and both treatments protectedIl1rl2−/−mice from uncontained infection. Furthermore, IL-36R-mediated IL-22 production by CD4+T cells was dependent upon NFκB-p65 and IL-6 expression in dendritic cells (DCs), as well as aryl hydrocarbon receptor (AhR) expression by CD4+T cells. Collectively, these data demonstrate that the IL-36 signaling pathway integrates innate and adaptive immunity leading to host defense against enteropathogenic bacterial infection.

Funder

HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases

Publisher

Proceedings of the National Academy of Sciences

Subject

Multidisciplinary

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